Journal: Frontiers in Microbiology

Article Title: Effect of biofilm formation on the antimicrobial activity of tigecycline against Mycobacterium abscessus in the hollow fiber infection model

doi: 10.3389/fmicb.2026.1799565

Figure Lengend Snippet: Schematic representation of applied in vitro model systems. (A) In Vitro Hollow Fiber Infection Model (HFIM) experimental system; and (B) Transwell™-based permeability assay platform. ECS extracellular capillary space; ICS intracellular capillary space.

Article Snippet: To perform dynamic time-kill assay experiments under biologically meaningful conditions, drug exposure mimicking in vivo lung PK profiles obtained after IPA administration of TGC in mice was experimentally simulated in the HFIM (C3008, FiberCell Systems, Frederick, MD).

Techniques: In Vitro, Infection, Permeability

Journal: Frontiers in Microbiology

Article Title: Effect of biofilm formation on the antimicrobial activity of tigecycline against Mycobacterium abscessus in the hollow fiber infection model

doi: 10.3389/fmicb.2026.1799565

Figure Lengend Snippet: Bacterial killing of Mab in the hollow fiber infection model (HFIM). (A) Pharmacokinetic Comparison: The in vivo concentration-time profiles of TGC in mouse lungs following 10 mg/kg intrapulmonary aerosol (IPA) administration (mean ± SD) in comparison to the in vitro concentration-time profile simulated using the HFIM after a direct injection of 600 μg TGC into the peripheral compartment. (B) Time courses of bacterial counts in CFU/mL in the HFIM cartridge compartment assessed under the various TGC exposures achieved through once-daily direct injection of different TGC doses into the cartridge compartment for 7 days. Symbols and lines represent observed bacterial counts (mean ± SD) and model-based simulation profiles, respectively. BF-embedded Mab is indicated by a dashed line with triangle symbols. Each set of triplicate experimental results was individually captured by corresponding model-based simulation profiles.

Article Snippet: To perform dynamic time-kill assay experiments under biologically meaningful conditions, drug exposure mimicking in vivo lung PK profiles obtained after IPA administration of TGC in mice was experimentally simulated in the HFIM (C3008, FiberCell Systems, Frederick, MD).

Techniques: Infection, Comparison, In Vivo, Concentration Assay, Aerosol, In Vitro, Injection

Journal: Frontiers in Microbiology

Article Title: Effect of biofilm formation on the antimicrobial activity of tigecycline against Mycobacterium abscessus in the hollow fiber infection model

doi: 10.3389/fmicb.2026.1799565

Figure Lengend Snippet: Effect of BF formation on TGC exposure and bacterial killing under various dose levels and treatment scenarios. (A) Longitudinal BF formation for different daily administered TGC doses simulated with the PK/PD model. (B) PK/PD-model simulated TGC concentration-time profiles within the BF compartment when once daily TGC dosing was initiated without delay following Mab inoculation. (C) PK/PD-model simulated TGC concentration-time profiles within the BF compartment when once daily TGC dosing was initiated once daily with 2-week delay following Mab inoculation, i.e., when a mature BF has developed. (D) Bacterial killing effect of high-dose TGC administered to the HFIM cartridge after a mature BF has been established. The dashed horizontal red lines in panels B and C indicate the half-maximal bacterial killing concentration for less susceptible populations of Mab (Mab-ls KC 50 ).

Article Snippet: To perform dynamic time-kill assay experiments under biologically meaningful conditions, drug exposure mimicking in vivo lung PK profiles obtained after IPA administration of TGC in mice was experimentally simulated in the HFIM (C3008, FiberCell Systems, Frederick, MD).

Techniques: Concentration Assay